Are you curious to know what is TLC test? You have come to the right place as I am going to tell you everything about TLC test in a very simple explanation. Without further discussion let’s begin to know what is TLC test?
TLC test, or Thin Layer Chromatography test, is a simple and inexpensive analytical technique used to separate and identify individual components of a mixture. This technique is widely used in chemistry, biochemistry, and pharmaceuticals, as well as in forensic science. In this blog, we will explore more about the TLC test, its principles, and its applications.
What Is TLC Test?
TLC test is a type of chromatography technique that uses a stationary phase, typically a thin layer of silica gel or alumina, and a mobile phase, which is usually a liquid solvent or a mixture of solvents. The mixture is applied to the stationary phase, and the mobile phase is allowed to move through it by capillary action. As the mobile phase moves, the individual components of the mixture separate and form distinct bands on the stationary phase.
Principles Of TLC Test:
The separation of components in a TLC test is based on their differential adsorption and partitioning between the stationary and mobile phases. The stationary phase is polar, while the mobile phase is non-polar. As a result, the more polar components of the mixture are adsorbed more strongly onto the stationary phase, while the less polar components remain in the mobile phase and move faster.
Applications Of TLC Test:
TLC test is a versatile analytical technique that finds numerous applications in various fields. Some of the most common applications of TLC test include:
- Separation and identification of components in a mixture.
- Purity determination of a compound.
- Quality control of pharmaceuticals.
- Analysis of natural products, such as plant extracts.
- Detection and quantification of drugs in forensic science.
- Analysis of food and beverage additives.
Conclusion:
In conclusion, TLC test is a powerful analytical technique that is widely used to separate and identify individual components of a mixture. Its simplicity, speed, and low cost make it an attractive choice for various applications in chemistry, biochemistry, and pharmaceuticals, as well as in forensic science. Whether you are a student, a researcher, or a scientist, the TLC test is a technique that you should be familiar with.
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FAQ
What Happens If TLC Is High?
What happens if the TLC count is high? A high TLC level in the blood indicates a possible risk of infection in the body. However, that’s not all because several undiagnosed medical conditions can elevate leukocyte levels too.
What Is The TLC Test Normal Range?
What is the normal range for TLC Test? The normal range of TLC is between 4000 to 11,000/microliters in an adult individual.
What Is TLC In Blood Test Low?
Why is TLC done? The Total Leukocyte Count Test is performed: As a part of the Complete Blood Count (CBC) Test in regular health checkups. To help diagnose infections and inflammation. To help diagnose conditions that decrease WBC count like bone marrow disorders.
What Is The TLC Blood Test Used For?
TLC or WBC count is a blood test that evaluates the number of WBC (white blood cells), also known as leukocytes. The leukocytes are the essential parts of our defense mechanism as they fight infections.
What Is A Normal TLC Count?
Total leukocytes (adults and children >2 years) – 5000-10,000/mm. Total leukocytes (children aged 2 years or below) – 6200-17,000/mm. Total leukocytes (neonates) – 9000-30,000/mm.
What Should I Eat If My TLC Is Low?
The TLC diet recommends limiting serving sizes or replacing foods high in saturated fat and cholesterol with healthier options. Fruits, vegetables, legumes (beans and lentils), nuts, whole grains, low- or non-fat dairy products, fish, poultry without the skin, and in moderate amounts, lean meats are good options.
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